https://doi.org/10.4081/jlimnol.2024.2187
Study on the immune enhancers against Micropterus salmoides rhabdovirus infection
Submitted: 14 March 2024
Accepted: 25 July 2024
Published: 11 October 2024
Accepted: 25 July 2024
Abstract Views: 2628
PDF: 41
HTML: 0
HTML: 0
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Micropterus salmoides rhabdovirus (MSRV) is one of the most serious pathogens harming M. salmoides juvenile, which had brought huge economic losses to farming industry. Studies involving candidate genes to the clinical diseases, however, are limited. In this study, the viral target and clinical manifestation of MSRV on M. salmoides juvenile were analyzed, and the protective effects of a single immune enhancer and a compound immune enhancer were evaluated. The results showed that the brain, liver, intestine and muscle of M. salmoides showed obvious lesions after infection with MSRV. The relative expression levels of nucleoprotein (N) and matrix protein (M) genes showed a trend of increasing at first and then decreasing and reached the peak in each tissue at 36 h post-infection. The mortality rate of M. salmoides was over 90% after 7 days of MSRV infection. The immune enhancers containing free nucleotides and Astragalus polysaccharide added to the diet effectively inhibited the replication of N and M genes in M. salmoides and increased the survival rate by 25% to 28%. This study provided basic data and theoretical reference for the analysis of the pathological mechanism and prevention and treatment of MSRV.
Aggad D, Stein C, Sieger D, Mazel M, Boudinot P, Herbomel P, et al. 2010. In vivo analysis of ifn-γ1 and ifn-γ2 signaling in zebrafish. J Immunol 185:6774-6782.
DOI: https://doi.org/10.4049/jimmunol.1000549
Awad E, Awaad A, 2017. Role of medicinal plants on growth performance and immune status in fish. Fish Shellfish Immunol 67:40-54.
DOI: https://doi.org/10.1016/j.fsi.2017.05.034
Betts AM, Stone DM, Way K, Torhy C, Chilmonczyk S, Benmansour A, de Kinkelin P, 2003. Emerging vesiculo-type virus infections of freshwater fishes in Europe. Dis Aquat Organ 57:201-212.
DOI: https://doi.org/10.3354/dao057201
Bin H, Liping S, Shuquan M, Peng X, 2019. Effects of four Chinese herbal preparations on growth performance and antioxidant activity in juvenile Micropterus salmoides. J Guangdong Ocean Univ 39:101-107.
Choi TY, Choi TI, Lee YR, Choe SK, Kim CH, 2021. Zebrafish as an animal model for biomedical research. Exp Mol Med 53:310-317.
DOI: https://doi.org/10.1038/s12276-021-00571-5
Fu X, Lin Q, Liang H, Liu L, Huang Z, Li N, Su J, 2017. The biological features and genetic diversity of novel fish rhabdovirus isolates in China. Arch Virol 162:2829-2834.
DOI: https://doi.org/10.1007/s00705-017-3416-z
Gao EB, Chen G, 2018. Micropterus salmoides rhabdovirus (MSRV) infection induced apoptosis and activated interferon signaling pathway in largemouth bass skin cells. Fish Shellfish Immunol 76:161-166.
DOI: https://doi.org/10.1016/j.fsi.2018.03.008
Guo Z, Zhao Z, Zhang C, Jia Y, Qiu D, Zhu B, Wang G, 2020. Carbon nanotubes-loaded subunit vaccine can increase protective immunity against rhabdovirus infections of largemouth bass (Micropterus salmoides). Fish Shellfish Immunol 99:548-554.
DOI: https://doi.org/10.1016/j.fsi.2020.02.055
Hussein GHG, Chen M, Qi PP, Cui QK, Yu Y, Hu WH, et al., 2020. Aquaculture industry development, annual price analysis and out-of-season spawning in largemouth bass Micropterus salmoides. Aquaculture 519:734901.
DOI: https://doi.org/10.1016/j.aquaculture.2019.734901
Iverson LE, Rose JK, 1981. Localized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription. Cell 23:477-484.
DOI: https://doi.org/10.1016/0092-8674(81)90143-4
Levraud J-P, Boudinot P, Colin I, Benmansour A, Peyrieras N, Herbomel P, Lutfalla G, 2007. Identification of the zebrafish ifn receptor: implications for the origin of the vertebrate ifn system. J Immunol 178:4385-4394.
DOI: https://doi.org/10.4049/jimmunol.178.7.4385
Gui J, Zhou L, Zhang X, 2018. Research advances and prospects for fish genetic breeding. Bull Chinese Acad Sci 33:932-939.
Kang SY, Kang JY, Oh MJ, 2012. Antiviral activities of flavonoids isolated from the bark of rhus verniciflua stokes against fish pathogenic viruses in vitro. J Microbiol 50:293-300.
DOI: https://doi.org/10.1007/s12275-012-2068-7
Kavaliauskis A, Arnemo M, Kim S-H, Ulanova L, Speth M, Novoa B, et al., 2015. Use of poly(i:c) stabilized with chitosan as a vaccine-adjuvant against viral hemorrhagic septicemia virus infection in zebrafish. Zebrafish 12:421-431.
DOI: https://doi.org/10.1089/zeb.2015.1126
Kavaliauskis A, Arnemo M, Speth M, Lagos L, Rishovd A, Estepa A, et al., 2016. Protective effect of a recombinant vhsv-g vaccine using poly(i:c) loaded nanoparticles as an adjuvant in zebrafish (Danio rerio) infection model. Dev Comp Immunol 61:248-257.
DOI: https://doi.org/10.1016/j.dci.2016.04.010
Kim SY, Kim SR, Oh MJ, Jung SJ, Kang SY, 2011. In vitro antiviral activity of red alga, polysiphonia morrowii extract and its bromophenols against fish pathogenic infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus. J Microbiol 49:102-106.
DOI: https://doi.org/10.1007/s12275-011-1035-z
Kumar S, Choubey AK, Srivastava PK, 2022. The effects of dietary immunostimulants on the innate immune response of Indian major carp: a review. Fish Shellfish Immunol 123:36-49.
DOI: https://doi.org/10.1016/j.fsi.2022.02.039
Lang G, Qiya Z, 2019. A brief review of aquatic animal virology research in China. J Fisher China 43:168-187.
Lei Y, Qi R, Cui L, Xiao Y, Zhang W, Ma J, Wang X, 2015. Diagnosis of rhabdovirus disease in juvenile largemouth bass Micropterus salmoides. J Dalian Ocean Univ 30:305-308.
Li BY, Yang F, Zhang ZY, Shen YF, Wang T, Zhao L, et al., 2022. Quinoline, with the active site of 8-hydroxyl, efficiently inhibits Micropterus salmoides rhabdovirus (MSRV) infection in vitro and in vivo. J Fish Dis 45:895-905.
DOI: https://doi.org/10.1111/jfd.13615
Li S, Chi SY, Cheng X, Wu C, Xu Q, Qu P, et al., 2020. Effects of antimicrobial peptides on the growth performance, antioxidant and intestinal function in juvenile largemouth bass, Micropterus salmoides. Aquacult Rep 16:100252.
DOI: https://doi.org/10.1016/j.aqrep.2019.100252
Li S, Lian X, Chen N, Wang M, Sang C, 2018. Effects of dietary vitamin e level on growth performance, feed utilization, antioxidant capacity and nonspecific immunity of largemouth bass, Micropterus salmoides. Aquacult Nutr 24:1679-1688.
DOI: https://doi.org/10.1111/anu.12802
Lin SM, Jiang Y, Chen YJ, Luo L, Doolgindachbaporn S, Yuangsoi B, 2017. Effects of astragalus polysaccharides (aps) and chitooligosaccharides (cos) on growth, immune response and disease resistance of juvenile largemouth bass, Micropterus salmoides. Fish Shellfish Immunol 70:40-47.
DOI: https://doi.org/10.1016/j.fsi.2017.08.035
Lin SM, Shi CM, Mu MM, Chen YJ, Luo L, 2018. Effect of high dietary starch levels on growth, hepatic glucose metabolism, oxidative status and immune response of juvenile largemouth bass, Micropterus salmoides. Fish Shellfish Immunol 78:121-126.
DOI: https://doi.org/10.1016/j.fsi.2018.04.046
Lin SM, Zhou XM, Zhou YL, Kuang WM, Chen YJ, Luo L, Dai FY, 2020. Intestinal morphology, immunity and microbiota response to dietary fibers in largemouth bass, Micropterus salmoide. Fish Shellfish Immunol 103:135-142.
DOI: https://doi.org/10.1016/j.fsi.2020.04.070
López-Muñoz A, Roca FJ, Sepulcre MP, Meseguer J, Mulero V, 2009. Zebrafish larvae are unable to mount a protective antiviral response against waterborne infection by spring viremia of carp virus. Dev Comp Immunol 34:546-552.
DOI: https://doi.org/10.1016/j.dci.2009.12.015
Lu J, Luo S, Tang H, Liang J, Zhao Y, Hu Y, et al., 2023. Micropterus salmoides rhabdovirus enters cells via clathrin-mediated endocytosis pathway in a pH-, dynamin-, microtubule-, rab5-, and rab7-dependent manner. J Virol 97:714-723.
DOI: https://doi.org/10.1128/jvi.00714-23
Luo S, Liang J, Yang G, Lu J, Chen J, 2024. The laminin receptor is a receptor for Micropterus salmoides rhabdovirus. J Virol 98:e00697-24.
DOI: https://doi.org/10.1128/jvi.00697-24
Lyu SJ, Yuan XM, Zhang HQ, Shi WD, Hang XY, Liu L, Wu YL, 2019. Isolation and characterization of a novel strain (yh01) of Micropterus salmoides rhabdovirus and expression of its glycoprotein by the baculovirus expression system. J Zhejiang Univ Sci B 20:728-739.
DOI: https://doi.org/10.1631/jzus.B1900027
Ma D, Deng G, Bai J, Li S, Yu L, Quan Y, et al., 2013. A strain of Siniperca chuatsi rhabdovirus causes high mortality among cultured largemouth bass in south China. J Aquat Anim Health 25:197-204.
DOI: https://doi.org/10.1080/08997659.2013.799613
Novoa B, Romero A, Mulero V, Rodríguez I, Fernández I, Figueras A, 2006. Zebrafish (Danio rerio) as a model for the study of vaccination against viral haemorrhagic septicemia virus (vhsv). Vaccine 24:5806-5816.
DOI: https://doi.org/10.1016/j.vaccine.2006.05.015
Phelan PE, Pressley ME, Witten PE, Mellon MT, Blake S, Kim CH, 2005. Characterization of snakehead rhabdovirus infection in zebrafish (Danio rerio). J Virol 79:1842-1852.
DOI: https://doi.org/10.1128/JVI.79.3.1842-1852.2005
Rodríguez Saint-Jean S, De las Heras A, Carrillo W, Recio I, Ortiz-Delgado JB, Ramos M, et al., 2013. Antiviral activity of casein and αs2 casein hydrolysates against the infectious haematopoietic necrosis virus, a rhabdovirus from salmonid fish. J Fish Dis 36: 467-481.
DOI: https://doi.org/10.1111/j.1365-2761.2012.01448.x
Sakai M, 1999. Current research status of fish immunostimulants. Aquaculture 172:63-92.
DOI: https://doi.org/10.1016/S0044-8486(98)00436-0
Sanders GE, Batts WN, Winton JR, 2003. Susceptibility of zebrafish (Danio rerio) to a model pathogen, spring viremia of carp virus. Comp Med 53:514-521.
Shen YF, Liu YH, Li BY, Liu TQ, Wang GX, 2020. Evaluation on antiviral activity of a novel arctigenin derivative against multiple rhabdoviruses in aquaculture. Virus Res 285:198019.
DOI: https://doi.org/10.1016/j.virusres.2020.198019
Song Y, Hu X, Lü A, Sun J, Liu Y, Pei C, Li L, 2018. [Zebrafish as a model for Rhabdovirus infection and molecular mechanism of the immune response].[Article in Chinese with English abstract]. Sci Sinica Vitae 48:745-759.
DOI: https://doi.org/10.1360/N052017-00185
Xiaoying H, Xuemei Y, Sunjian L, Haiqi Z, Li L, Zhe Y, Weida S, 2021. [Screening and antiviral effect of Chinese herbal medicines against largmouth sea bass flavivirus].[Article in Chinese]. Jiangsu J Agric Sci 49:155-159.
Yan L, 2015. [Epidemiological characteristics and comprehensive prevention and control techniques of seabass flavivirus disease in California].[Article in Chinese]. Contemp Fish China 40:76.
Yang B, Guo ZR, Zhao Z, Wang T, Yang F, Ling F, et al. 2022. Protective immunity by DNA vaccine against Micropterus salmoides rhabdovirus. J Fish Dis 45:1429-1437.
DOI: https://doi.org/10.1111/jfd.13672
Yang F, Song K, Zhang Z, Chen C, Wang GX, et al., 2021. Evaluation on the antiviral activity of ribavirin against micropterus salmoides rhabdovirus (msrv) in vitro and in vivo. Aquaculture 543:736975.
DOI: https://doi.org/10.1016/j.aquaculture.2021.736975
Yusuf A, Huang XX, Chen N, Apraku A, Wang W, Cornel A, Rahman MM, 2020. Impact of dietary vitamin c on plasma metabolites, antioxidant capacity and innate immunocompetence in juvenile largemouth bass, Micropterus salmoides. Aquacult Rep 17:100383.
DOI: https://doi.org/10.1016/j.aqrep.2020.100383
Yutang W, 2017. [Species and research progress of fish immune enhancers (I)].[Article in Chinese]. Fish China 11:72-74.
Zhang L, Li N, Lin Q, Liu L, Liang H, Huang Z, Fu X, 2018. An avirulent Micropterus salmoides rhabdovirus vaccine candidate protects Chinese perch against rhabdovirus infection. Fish Shellfish Immunol 77:474-480.
DOI: https://doi.org/10.1016/j.fsi.2018.03.047
Zhang D, Yan H, Luo M, Yang Y, Hu Y, Gong C, et al., 2022. Effects of dietary Bacillus subtilis on intestinal structure, antioxidant capacity, immune capacity and enteritis of largemouth bass. Chin J Anim Nutr 34:575-588.
Edited by
Andrea Di Cesare, National Research Council, Water Research Institute (CNR-IRSA), Verbania Pallanza, ItalySupporting Agencies
Key Research and Development Program of Zhejiang Province, Key project of Xingtai University , Science and Technology Project of Zhejiang ProvinceHow to Cite
Lei, Ning, Chaonan Zhang, Yanchao Wang, and Junjie Zhu. 2024. “Study on the Immune Enhancers Against <i>Micropterus salmoides< i> Rhabdovirus Infection”. Journal of Limnology 83 (1). https://doi.org/10.4081/jlimnol.2024.2187.
Copyright (c) 2024 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Similar Articles
- Adrianna Wojtal-Frankiewicz, Joanna Bernasińska, Tomasz Jurczak, Krzysztof Gwoździński, Piotr Frankiewicz, Marzena Wielanek, Microcystin assimilation and detoxification by Daphnia spp. in two ecosystems of different cyanotoxin concentrations , Journal of Limnology: Vol. 72 No. 1 (2013)
- Ana Sanz, Manuel J. López-Rodríguez, Sergio García-Mesa, Cristina Trenzado, Rosa M. Ferrer, J. Manuel Tierno de Figueroa, Are antioxidant capacity and oxidative damage related to biological and autecological characteristics in aquatic insects? , Journal of Limnology: Vol. 76 No. 1 (2017)
- Wei Han, Yuanshu Jing, Ting Li, Compensatory growth in Microcystis aeruginosa after moderate high-temperature exposure , Journal of Limnology: Vol. 74 No. 3 (2015)
- Xiangyu Mu, James Brower, Donald I. Siegel, Anthony J. Fiorentino II, Shuqing An, Ying Cai, Delin Xu, Hao Jiang, Using integrated multivariate statistics to assess the hydrochemistry of surface water quality, Lake Taihu basin, China , Journal of Limnology: Vol. 74 No. 2 (2015)
- Stefan Sommer, Roberta Piscia, Marina M. Manca, Diego Fontaneto, Arpat Ozgul, Demographic cost and mechanisms of adaptation to environmental stress in resurrected Daphnia , Journal of Limnology: Vol. 75 No. s2 (2016): Lake Orta: a new lease on life
- Francesca Bona, Valentina La Morgia, Stefano Fenoglio, Luana Morandi, Elisa Falasco, Diatom communities and ecological status classification in the upper Po River basin , Journal of Limnology: Vol. 80 No. 3 (2021): Celebratory Issue - 80th Anniversary of the Journal of Limnology
- Irina P. Chubarenko, Elena Esiukova, Natalia Stepanova, Boris Chubarenko, Henning Baudler, Down-slope cascading modulated by day/night variations of solar heating , Journal of Limnology: Vol. 72 No. 2 (2013)
- Pietro Volta, Nicoletta Riccardi, Rosaria Lauceri, Mauro Tonolla, Discrimination of freshwater fish species by Matrix-Assisted Laser Desorption/Ionization- Time Of Flight Mass Spectrometry (MALDI-TOF MS): a pilot study , Journal of Limnology: Vol. 71 No. 1 (2012)
- Ambrosius J.M. Dörr, Antonia C. Elia, Marinella Rodolfi, Laura Garzoli, Anna M. Picco, Manuela D'Amen, Massimilaino Scalici, A model of co-occurrence: segregation and aggregation patterns in the mycoflora of the crayfish Procambarus clarkii in Lake Trasimeno (central Italy) , Journal of Limnology: Vol. 71 No. 1 (2012)
- Daniela Lupi, Anna Rocco, Bruno Rossaro, Benthic macroinvertebrates in Italian rice fields , Journal of Limnology: Vol. 72 No. 1 (2013)
You may also start an advanced similarity search for this article.
