https://doi.org/10.4081/jlimnol.2023.2087
Metabarcoding to monitor the crustacean zooplankton of a lake improves when using a reference DNA library from local samples
Submitted: 7 July 2022
Accepted: 16 January 2023
Published: 8 February 2023
Accepted: 16 January 2023
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Biodiversity surveys through morphology provide invaluable data to inform biological monitoring efforts, involving specialised taxonomic skills that are not always available. The revolution brought by the advent of metabarcoding associated to massive sequencing is currently seen as a potential advance, even if different approaches may often provide different results. Here we test if reliable results from metabarcoding can be obtained by i) basing the analyses on a detailed knowledge of the local diversity from morphology, ii) applying tools from DNA taxonomy to create a local reference library, ii) developing custom primers, taking as example the crustacean zooplankton of a subalpine lake in Northern Italy, Lake Maggiore. We support the idea that occurrences from metabarcoding can be reliable, especially with targeted primers, but we confirm that read numbers from massive sequencing could not be related to abundance of individuals in our analyses. Data from metabarcoding can thus be used to reliably monitor species occurrence in the lake, but not changes in abundance.
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Supporting Agencies
CIPAIS, Progetti@CNR SOS AcqueHow to Cite
Garlasché, Giuseppe, Giulia Borgomaneiro, Roberta Piscia, Marina Manca, Ester M. Eckert, and Diego Fontaneto. 2023. “Metabarcoding to Monitor the Crustacean Zooplankton of a Lake Improves When Using a Reference DNA Library from Local Samples”. Journal of Limnology 82 (1). https://doi.org/10.4081/jlimnol.2023.2087.
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