Chlorophyll a interference in phycocyanin and allophycocyanin spectrophotometric quantification

DOI https://doi.org/10.4081/jlimnol.2017.1691

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Published: Sep 28, 2017
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Vol 77 No 1 (2018)
Keywords
Cyanobacteria, phycocyanin, allophycocyanin, chlorophyll a, spectrophotometric equation.
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Lauceri, R., Bresciani, M., Lami, A., & Morabito, G. (2017). Chlorophyll a interference in phycocyanin and allophycocyanin spectrophotometric quantification. Journal of Limnology, 77(1). https://doi.org/10.4081/jlimnol.2017.1691
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Rosaria Lauceri *
National Research Council of Italy, Institute for the Study of Ecosystems, Italy.
Mariano Bresciani
National Research Council of Italy, Institute for Electromagnetic Sensing of the Environment, Optical Sensing Group, Italy.
Andrea Lami
National Research Council of Italy, Institute for the Study of Ecosystems, Italy.
Giuseppe Morabito
National Research Council of Italy, Institute for the Study of Ecosystems, Italy.
(*) Corresponding Author:
Rosaria Lauceri | r.lauceri@ise.cnr.it

Abstract

The accurate quantification of cyanobacteria phycobiliproteins is an important aspect in various research topics, such as cyanobacteria ecology and physiology studies, and especially to calibrate algorithms used in remote sensing of cyanobacterial blooms. Here we present a spectroscopic approach, exploiting spectrophotometric equations, aimed at improving the phycocyanin and allophycocyanin quantification when chlorophyll a is present in the phycobiliprotein aqueous extract.

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