Effect of starvation and subsequent feeding on glycogen concentration, behavior and mortality in the golden mussel Limnoperna fortunei (Dunker, 1857) (Bivalvia: Mytilidae)

  • Nelmara I.S. Cordeiro Universidade Federal de Minas Gerais, Brazil.
  • Jennifer T.M. Andrade Universidade Federal de Minas Gerais, Brazil.
  • Lângia C. Montresor FIOCRUZ, Brazil.
  • Dalva M.R. Luz LELf Centro de Pesquisas Hidráulicas e Recursos Hídricos, Brazil.
  • Carlos B. Martinez Universidade Federal de Minas Gerais, Brazil.
  • Gustavo Darrigran Consejo Nacional de Investigaciones Cientificas y Tecnicas-Argentina – CONICET, Argentina.
  • Jairo Pinheiro Universidade Federal Rural do Rio de Janeiro, Brazil.
  • Teofânia H.D.A. Vidigal | teofania.vidigal@gmail.com Universidade Federal de Minas Gerais, Brazil.

Abstract

The success of Limnoperna fortunei as an invasive species is related to its physiological plasticity that allows them to endure adverse environmental conditions. Starvation tolerance is considered to be an important trait associated with bivalve invasiveness. In natural ecosystems, food resources can vary during the year, exposing mussels to variable periods of starvation or limited food availability. Thus, mussels have developed physiological strategies to tolerate and survive fluctuations in food availability. Glycogen concentration has been used in different monitoring studies as an indicator of the nutritional condition of bivalves. The aim of this study was to investigate the physiological responses of L. fortunei based on the glycogen concentrations of specimens under four treatments, comprising different combinations of feeding and starvation, during 125 days. The experiment was carried out in two phases. In the phase I, mussels were divided in two treatments: starvation (S) and feeding (F). After 100 days, tissue samples were collected to quantify glycogen concentrations and, each phase I group was divided in two subgroups: starvation (S) and feeding (F), resulting in four treatments. In the phase II, that lasted 25 days, starvation specimens (S) from phase I were allowed to feed (starvation-feeding treatment , or S-F), or continued to undergo starvation (starvation-starvation treatment , or S-S) and the feeding specimens (F) continued feeding (feeding-feeding group, or F-F), or were subjected to starvation (feeding-starvation treatment , or F-S). Behavior (valve-closing) and mortality were recorded in 24 h intervals. After the 25 days (phase II) all specimens were killed, and their soft tissue was removed to quantify glycogen concentrations. The glycogen concentration of the S-F treatment was lower than that of the F-S treatment, which was initially allowed to feed (phase I) and then subjected to starvation (phase II). Stability in the glycogen concentrations was observed when the phase II feeding conditions were maintained during the experiments, as observed in the S-S (continued starvation) and F-F (continued feeding) treatments. Based on our glycogen concentrations results, the golden mussel shows a higher tolerance to starvation (125 days) than has previously been published, which suggests that its tolerance strongly influences its invasive behavior.

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Author Biographies

Nelmara I.S. Cordeiro, Universidade Federal de Minas Gerais
Departamento de Zoologia Laboratório de Estudos de Limnoperna fortunei – LELf Centro de Pesquisas Hidráulicas e Recursos Hídricos, Master
Jennifer T.M. Andrade, Universidade Federal de Minas Gerais

Laboratório de Malacologia e Sistemática Molecular - Programa de Pós-Graduação em Zoologia- Departamento de Zoologia, Ph.D student

Lângia C. Montresor, FIOCRUZ
Laboratório de Malacologia -  Instituto Oswaldo Cruz, Fundação Oswaldo Cruz - FIOCRUZ,  Dr
Dalva M.R. Luz, LELf Centro de Pesquisas Hidráulicas e Recursos Hídricos
Departamento de Zoologia, Laboratório de Estudos de Limnoperna fortunei
Carlos B. Martinez, Universidade Federal de Minas Gerais
Laboratório de Estudos de Limnoperna fortunei – LELf Centro de Pesquisas Hidráulicas e Recursos Hídricos, Universidade Federal de Minas Gerais, Dr
Gustavo Darrigran, Consejo Nacional de Investigaciones Cientificas y Tecnicas-Argentina – CONICET
Divisíon Zoología Invertebrados, Facultad Ciencias Naturales y Museo La Plata, Dr
Jairo Pinheiro, Universidade Federal Rural do Rio de Janeiro
Departamento de Ciências Fisiológicas, Instituto de Biologia, Dr
Teofânia H.D.A. Vidigal, Universidade Federal de Minas Gerais
Laboratório de Malacologia e Sistemática Molecular, Departamento de Zoologia Laboratório de Estudos de Limnoperna fortunei – LELf Centro de Pesquisas Hidráulicas e Recursos Hídricos, Dr
Published
2016-07-13
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Issue
Section
Original Articles
Supporting Agencies
Foundation for research from the State of Minas Gerais (Fapemig) and Vale S.A (CRA - RDP-00097-10), CNPq and Capes.
Keywords:
Bioinvasion, glycogen, golden mussel, starvation, comparative physiology.
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How to Cite
1.
Cordeiro NI, Andrade JT, Montresor LC, Luz DM, Martinez CB, Darrigran G, Pinheiro J, Vidigal TH. Effect of starvation and subsequent feeding on glycogen concentration, behavior and mortality in the golden mussel Limnoperna fortunei (Dunker, 1857) (Bivalvia: Mytilidae). J Limnol [Internet]. 2016Jul.13 [cited 2021May9];75(3). Available from: https://jlimnol.it/index.php/jlimnol/article/view/jlimnol.2016.1465